Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues. ==Mechanism== Phenylisothiocyanate is reacted with an uncharged terminal amino group, under mildly...
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http://en.wikipedia.org/wiki/Edman_degradation
(from the article `protein`) ...-amino group (NH2) of the N-terminal amino acid with phenyl isothiocyanate; subsequent mild hydrolysis does not affect the peptide bonds; the ...
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http://www.britannica.com/eb/a-z/e/11
A lab technique used to find out the order of amino acids in a polypeptide (chain of amino acids). It involves using the Edman reagent, phenyl isothiocyanate (PITC), to react one by one with each amino acid, in order. The technique is used in machines which automatically sequence (determine the order of subunits) polypeptides. ... (09 Oct 1997) ......
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http://www.encyclo.co.uk/local/20973
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